Products · AO software

Phinden

Phaseform's adaptive optics software. It measures and corrects aberrations directly from the images you acquire, in real time — running alongside the microscope control software you already use.

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Phinden's Corrections Control window: a Laplace-transformed image-quality metric over a plant-tissue field, with the aberration-selection and apply-correction controls

Sensorless correction software

Aberration correction in a few clicks

Phinden measures and corrects aberrations from the frames you already acquire — no wavefront sensor, no additional hardware, no modification to the microscope.

Phinden estimates the aberrations from the image, applies the correction through the Deformable Phase Plate (DPP) and uses image-quality metrics to guide the optimization — during live acquisition, in the background of your microscope software.

Every Delta 7 ships with basic control software for driving the DPP directly. Phinden is the layer above it, finding the correction from the image itself instead of leaving you to dial in modes by hand.

Automated measurement and correctionImage-based adaptive optics at your fingertips — a few mouse clicks optimize image quality during live acquisition.
Plug-and-play adaptive opticsA streamlined path to full AO integration: Phaseform's DPP with minimal hardware and software setup.
Runs alongside your control softwareWorks in the background of platforms like µManager and ScanImage — you operate the microscope as usual.
Diverse modalities and specimensA broad selection of implemented image-quality metrics guides the optimal correction for a wide range of techniques and samples.

The sensorless loop: the image feeds the aberration estimate, the correction goes to the DPP, and the focus at the sample sharpens.

Pollen grain imaged with two-photon excitation, with Phinden's correction — Pollen grain, two-photon excitation through a custom cover glass. The corrected side gains contrast and brightness, and the finer structures become visible.Thorlabs Bergamo, DZNE Bonn, Light Microscopy Facility. Courtesy of Dr. Hans-Ulrich Fried.

The same pollen grain without adaptive optics — Pollen grain, two-photon excitation through a custom cover glass. The corrected side gains contrast and brightness, and the finer structures become visible.Thorlabs Bergamo, DZNE Bonn, Light Microscopy Facility. Courtesy of Dr. Hans-Ulrich Fried.

Demo

a complete brightfield run — from the first aberrated frame to the corrected image — recorded inside µManager

Download specifications How the DPP corrects wavefronts Talk to us about your microscope

Works inside your microscope software

You keep using your microscope software — Phinden talks to it and applies the correction through the DPP.

Phinden runs in the background alongside the native control software, acquires the frames it needs and applies the correction to the Delta 7 or PHI. It can also drive the DPP directly from Python. An integration with Nikon NIS-Elements is in preparation.

Phinden in the field

one installation per control software, at partner labs

Thorlabs Bergamo multiphoton microscope with the Delta 7 integrated below the scan head — ThorImageThorlabs Bergamo multiphoton microscopeDZNE Bonn, Light Microscopy Facility · Dr. Hans-Ulrich Fried

ThorImageThorlabs Bergamo multiphoton microscopeDZNE Bonn, Light Microscopy Facility · Dr. Hans-Ulrich Fried

Delta 7 in an INSS 2-photon confocal microscope — ScanImageINSS 2-photon confocal microscopeDZNE Bonn, Light Microscopy Facility · Dr. Hans-Ulrich Fried

Phaseform adaptive optics on the camera port of a ZEISS Axiovert 200M at the Life Imaging Center, University of Freiburg — ZEN blueZEISS Axiovert 200MLife Imaging Center, University of Freiburg · Dr. Roland Nitschke

ZEN blueZEISS Axiovert 200MLife Imaging Center, University of Freiburg · Dr. Roland Nitschke

Delta 7 in a light-sheet microscope — µManagerLight-sheet microscopeMedizinische Universität Wien · Prof. Hans-Ulrich Dodt

µManagerLight-sheet microscopeMedizinische Universität Wien · Prof. Hans-Ulrich Dodt

Ask about your control software Read about the ScanImage integration

Application example

Adaptive optics in a commercial multiphoton microscope

Two Delta 7s in the beam path of a Thorlabs Bergamo at the DZNE in Bonn — corrected by Phinden.

The two are a Delta 7-10 and a Delta 7-20, both below the scan head, integrated together with the team at the German Center for Neurodegenerative Diseases (DZNE) Light Microscopy Facility.

Delta 7-10 and Delta 7-20 mounted in the Bergamo beam path

Three-photon imaging

fixed mouse brain at 320 µm depth — Phinden driving ThorImage on the Bergamo

The same field with Phinden's correction applied — Fixed mouse brain 320 µm deep, three-photon (3P) imaging at 1300 nm — a 32 µm z-stack projection. Olympus 25×/1.05 W.Thorlabs Bergamo, DZNE Bonn, Light Microscopy Facility. Courtesy of Dr. Hans-Ulrich Fried.

Fixed mouse brain at 320 µm depth, three-photon, AO off — Fixed mouse brain 320 µm deep, three-photon (3P) imaging at 1300 nm — a 32 µm z-stack projection. Olympus 25×/1.05 W.Thorlabs Bergamo, DZNE Bonn, Light Microscopy Facility. Courtesy of Dr. Hans-Ulrich Fried.

Download the microscopy application note Discuss your multiphoton system

System requirements

Supported control software

Phinden runs alongside these packages — or is scripted directly from Python.

Software	Requirement
ThorImage	Version available upon request from Thorlabs
ZEN blue	Version: 3.2 – 3.10, Macro environment included
ScanImage	MATLAB R2020b or higher ScanImage Premium license, version 2019R0 to 2023.1.0 Windows 7 SP1, 8.1, 10, 11
µManager	µManager 2.0
SDK Python	Version 3.8 to 3.12
Next:NIS-Elements	Integration in preparation — supported versions to be confirmed

Your desired control software not listed?

Downloads

Spec sheet and brochures

PhindenSpec sheet↓ The Deformable Phase Plate TechnologyWhite paper↓ Refractive Adaptive Optics for MicroscopyApplication note↓ Refractive Adaptive Optics for OphthalmoscopyWhite paper↓

Specifications Request a quotation